phage-derived gamma polyglutamic acid hydrolase, sporulation protein
function
polyglutamic acid degradation
product
phage-derived gamma polyglutamic acid hydrolase
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.4|Capsule biosynthesis and degradation][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
1,915,221 → 1,915,979
The protein
Catalyzed reaction/ biological activity
hydrolysis of polyglutamic acid [Pubmed|26158264]Protein family
UPF0714 family (according to Swiss-Prot)Paralogous protein(s)
[protein|8F9DCD77660706E4D25CD7875C014F6A2B5D8123|PghZ], [protein|BA7D1D112E934B68073DFC1F76248ED6157240E3|PghB], [protein|DF95DED9A6E2160C151D7664C951B198EEA225E8|PghC] [SW|Domains]
DUF867 [http://pfam.xfam.org/family/PF05908 x], [[gamma-PGA hydrolase domain]] [Pubmed|26158264]Structure
[PDB|3A9L] (from B. subtilis phage NIT1, 36% identity) [pubmed|22105902][PDB|5ONJ] (5 amino acid complex)[PDB|6HRI] (native)[PDB|6HRJ] (apo-form)[SW|Localization]
cell membrane (according to Swiss-Prot)Expression and Regulation
Operons
genes
[gene|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL]
description
[Pubmed|16385044]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,16385044], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]regulation
expressed during [SW|sporulation] in the mother cell[Pubmed|16385044]additional information
An [ncRNA|search|antisense RNA], '[protein|search|surA]', is predicted for '[protein|search|pghL]' [PubMed|20525796]view in new tabBiological materials
Mutant
MGNA-B113 (yndL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1112 NBRP B. subtilis, Japan]BKE17820 (Δ[gene|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTACTCCGCCTCTAAGT, downstream forward: _UP4_TGAAAAATAGGAGGCGATAABKK17820 (Δ[gene|FD862CE6C4A37B17714F8F8687B3266D43EF5F63|pghL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTACTCCGCCTCTAAGT, downstream forward: _UP4_TGAAAAATAGGAGGCGATAAReferences
16385044,20525796,26158264,27766092,22105902